| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004388 |
| Chromosome: | chromosome 12 |
| Location: | 1998861 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510850 | CGL73 | expressed protein Conserved in the Green Lineage; (1 of 2) PF11833 - Protein of unknown function (DUF3353) (DUF3353) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACACAGCCGCAGGCCAGCCCCCGGAGAATACACAACGTGATGCGCTG |
| Internal bar code: | TCTCGTATTTTTTTGCTCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2449 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCCAGATGGAGACTACA |
| Suggested primer 2: | CGGTCATTTCCACCCCAGAA |