Insertion junction: CLIP2.004395_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004395
Chromosome:chromosome 3
Location:2099611
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156500 (1 of 1) IPR000104//IPR016084 - Antifreeze protein, type I // Haem oxygenase-like, multi-helical CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACGTGGCTGACCATCTTCAGCTCGCCCAGCTTCGACCGGTTGCCGTC
Internal bar code:ACCCGCAACTCTTGTCTGTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2750
LEAP-Seq percent confirming:97.7778
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCCATGTCACCACCTAGC
Suggested primer 2:CTCCAGGGCTACACACACAG