Insertion junction: CLIP2.004413_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004413
Chromosome:chromosome 2
Location:1114992
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g080700 BIP1 (1 of 2) K09490 - heat shock 70kDa protein 5 (HSPA5, BIP); Endoplasmic reticulum associated Hsp70 protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATCGACGCCCGCAACCAGCTGGAGACCTACTGCTACAACATGAAGAGC
Internal bar code:GTGCTGTATTAGCGAGGCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1739
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACATCCTACGAGTCGAGGC
Suggested primer 2:AGGACATCCTGCTCCTGGAT