Insertion junction: CLIP2.004426_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004426
Chromosome:chromosome 8
Location:2489199
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g372450 PSBQ1,OEE3, PSBQ (1 of 1) K08901 - photosystem II oxygen-evolving enhancer protein 3 (psbQ); Photosystem II Oxygen Evolution Enhancer protein 3 outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTAAGCTTGAGAATACACGTGGCAAACTGGGGTTGCCAAATGTGCGGA
Internal bar code:GGGTTAGCCGAGAGACGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTGTCGTGTCAACTCGG
Suggested primer 2:GGAAACTCGCAGTCAGGACA