| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004431 |
| Chromosome: | chromosome 7 |
| Location: | 1188919 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321250 | 3'UTR | ||
| Cre07.g321300 | (1 of 3) PF03703 - Bacterial PH domain (bPH_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGAAGAAAGATGGCTCTGCCTCTCAAAATTGGTTACAAGAGGGACCT |
| Internal bar code: | GAAGAGTTTACCCGGAGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 71.875 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCCACATGCTGTAGGTG |
| Suggested primer 2: | GCCACCATTCGCATGTGATC |