| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004451 |
| Chromosome: | chromosome 1 |
| Location: | 5432114 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038350 | (1 of 39) IPR009003 - Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGGAAGGGAGACTGTAGGCACCAGAGTTACCGGTGCAACTTTGGAAT |
| Internal bar code: | GGAAGTCTATCACTCGAGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5933 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTACCTCGCACCATAC |
| Suggested primer 2: | GATCCATTGCTAGCGGACGA |