| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.004458 |
| Chromosome: | chromosome 17 |
| Location: | 2021285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710800 | NFU3,NIFU3 | (1 of 3) PF01106 - NifU-like domain (NifU); iron-sulfur cluster assembly protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGCGTGGAGACACGGTGCCGGCAGCTATGGAGCTGTCGGTTGCGGTC |
| Internal bar code: | GAAAAAAGGGATATAGACGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1455 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCCATCAAGGTGGGATT |
| Suggested primer 2: | CACGTTTCAGGCACACACAG |