Insertion junction: CLIP2.004467_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.004467
Chromosome:chromosome 17
Location:1171358
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g704350 GLOD4 (1 of 1) PTHR10374//PTHR10374:SF20 - LACTOYLGLUTATHIONE LYASE GLYOXALASE I // GLYOXALASE DOMAIN-CONTAINING PROTEIN 4; Glyoxalase domain containing 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCCCTCTCATACAGGGTGTGAACGCGGACCGCATGCAGTCGCTGTCAA
Internal bar code:AGTATTTAGTATGTACGTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1119
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGGAGGAAGCACTGGAG
Suggested primer 2:CCTCCCATCAACCTTGCCAT