| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.004475 |
| Chromosome: | chromosome 2 |
| Location: | 6319797 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387550 | CGL142 | Conserved in the Green Lineage | CDS |
| Cre09.g387600 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGTGCGCTGTCCGATCGATTCGCGTCAAGTCAAATTGCCAACTCGC |
| Internal bar code: | CCTCGATGACGGAACAAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2100 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGCGACAAGACAAGTG |
| Suggested primer 2: | CGAACTGGTGTCAGACAGCT |