| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004492 |
| Chromosome: | chromosome 5 |
| Location: | 378880 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGAGCGCGGAGAGAGCCTTCAAAATCGGCTGGATGGAGGTTTCATTT |
| Internal bar code: | AGGGCTATACCTACGGTATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1559 |
| LEAP-Seq percent confirming: | 4.83871 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATATGAGTTCGGCGCACG |
| Suggested primer 2: | GGGGAGGACCGAAAGAAAGG |