Insertion junction: CLIP2.004494_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004494
Chromosome:chromosome 1
Location:3470015
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g022600 CGL13 Conserved in the Green Lineage; (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACATCGCACGTAAGCCCAGCCCGAGACCATTTCGACCACCACTGCGG
Internal bar code:AACTGCCACGGTGAGGTCCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1502
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCGTCATCCTCCTCATC
Suggested primer 2:GATCGAGGTGGAGCAGGATG