Insertion junction: CLIP2.004531_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.004531
Chromosome:chromosome 6
Location:2885135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272650 LHCA8 Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTCAGCCTCACCAAGGGGGTCGAAGGGGCCGCCGGGGTAGCCCAGCT
Internal bar code:GGTGGTCGATATATAAAGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1728
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGCAGTATGACAATGCG
Suggested primer 2:ACCGGGCATTTACAGGAAGG