Insertion junction: CLIP2.004534_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004534
Chromosome:chromosome 10
Location:28575
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g417550 CLPS3,CLPT3 (1 of 2) 3.6.4.10 - Non-chaperonin molecular chaperone ATPase / Molecular chaperone Hsc70 ATPase; Additional subunit of chloroplast ClpP complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAGCCTTTCCCATGTGGTCCTTCGGTGCCAGCTCGCTGCTTTCAACTT
Internal bar code:GGTAGTGCCCTTTTTAATGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2551
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTACACCAAGCGTTTTGC
Suggested primer 2:GATGTGGGTCTGTGGAAGGG