Insertion junction: CLIP2.004537_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004537
Chromosome:chromosome 1
Location:7741713
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g054500 (1 of 1) 1.6.1.2 - NAD(P)(+) transhydrogenase (Re/Si-specific) / Transhydrogenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGAGCTGAAGGCGGCCGAGGGCGAGGTGGCGCCCAGCGCCAGCGCGGC
Internal bar code:TTTTTTTAATACAGGTCCTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1612
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCAACAACATCTCCAAGC
Suggested primer 2:TCGCTTCTGTCCCCCAAATC