Insertion junction: CLIP2.004546_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004546
Chromosome:chromosome 1
Location:3202638
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g020400 (1 of 1) K13111 - WD40 repeat-containing protein SMU1 (SMU1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTGACGCGCACACGCACACACACCCACACACATACACACACGCACAT
Internal bar code:TACGCAAATTTGAGCTAATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1149
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACACATCGCATACACTG
Suggested primer 2:GTGGCCGACATAAACAACGG