Insertion junction: CLIP2.004548_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004548
Chromosome:chromosome 7
Location:1699861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACCAAATGATGACGAGGGGAACAAAGGTGCCCATCCACACCCGGAT
Internal bar code:TGTGAAATGGTGAACGGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:6386
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGCTACTGTGCCTAGGC
Suggested primer 2:ACTTTGGGTGTCAGGCCAAA