Insertion junction: CLIP2.004548_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004548
Chromosome:chromosome 7
Location:1699868
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGCCCCTGTTTGACGAGGGGCTCTCCAGGGGGGCGTAGAACCTTCCG
Internal bar code:TGTGAAATGGTGAACGGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1838
LEAP-Seq percent confirming:58.8235
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTTGGGTGTCAGGCCAAA
Suggested primer 2:ATGTGCTACTGTGCCTAGGC