| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004574 |
| Chromosome: | chromosome 2 |
| Location: | 1114991 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080700 | BIP1 | (1 of 2) K09490 - heat shock 70kDa protein 5 (HSPA5, BIP); Endoplasmic reticulum associated Hsp70 protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTTGACGGCCTTGTCCTGCTCCGCAAACTCCTCAGCCTCCTTCACCA |
| Internal bar code: | GCAGGTGCAGGTCGGTATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4272 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 156 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACATCCTGCTCCTGGAT |
| Suggested primer 2: | CACATCCTACGAGTCGAGGC |