Insertion junction: CLIP2.004575_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004575
Chromosome:chromosome 16
Location:4035071
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690130 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGACATGAGGAGACAGGCTCCGCCAGGCGCCACCAGTGCAAGCGCAA
Internal bar code:GTCATGTGAGTGCGCAAGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2912
LEAP-Seq percent confirming:98.0769
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:CACTACCTTTGCGGTTTGGC
Suggested primer 2:AAGGAGTAGCAGCACACACC