Insertion junction: CLIP2.004585_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.004585
Chromosome:chromosome 1
Location:7555726
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g053000 GPD1,GPDH2,GPD2 (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAACCATGCCTTCAGCACTCGTCAAATGAGCCACCAGCGCGGAGCGCT
Internal bar code:ATCGTAATGTTTGGACAGTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1841
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGCACACCTACACACACG
Suggested primer 2:TAAACGCCCTATCTTCCCGC