| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004619 |
| Chromosome: | chromosome 6 |
| Location: | 6815543 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296250 | SYK1,TSK2 | Putative organellar lysyl-tRNA synthetase, tRNA synthetase class II (D, K and N) family protein; (1 of 1) PTHR22594//PTHR22594:SF4 - ASPARTYL/LYSYL-TRNA SYNTHETASE // LYSINE--TRNA LIGASE-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGTTTTTTTGGGGGGAAGGGGAGGTTATGTTGTTCCGTTCCCAAAGA |
| Internal bar code: | TCTCAATGTTTGTGGCGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 45.7143 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTCCCACCTGGTAGATC |
| Suggested primer 2: | CTCCGTCGCTAGTTCGAGTC |