| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004627 |
| Chromosome: | chromosome 8 |
| Location: | 4236291 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGGATCCAGGCCGCACCGAGCCCAGCGGCGCGTAGGAGCCGCCGGCC |
| Internal bar code: | TCCCCCGTTTACTAGGGCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1728 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATACCGGAAGCGTGCAAAC |
| Suggested primer 2: | TGTTGCTGGTGTTGTTGCTG |