Insertion junction: CLIP2.004627_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.004627
Chromosome:chromosome 8
Location:4236301
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g384150 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATCCTCGTTGGACCCCGGTCTGGCCACCCCGCCGCTGTCGCTCTCCGG
Internal bar code:TCCCCCGTTTACTAGGGCGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:3030
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGCTGGTGTTGTTGCTG
Suggested primer 2:GATACCGGAAGCGTGCAAAC