| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004633 |
| Chromosome: | chromosome 4 |
| Location: | 3454974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227700 | 3'UTR | ||
| Cre04.g227750 | (1 of 1) PF08573 - DNA repair protein endonuclease SAE2/CtIP C-terminus (SAE2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCGGCAAGTGGTATGTCATCTTGTCTGTTCAAGTTCCAATTGCCCAT |
| Internal bar code: | GGATTTTGATTGATGTGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2242 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGTAGATGTCTGAGGCC |
| Suggested primer 2: | GGGCTACGGTGTCTATGAGC |