Insertion junction: CLIP2.004655_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.004655
Chromosome:chromosome 6
Location:5243737
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g284150 RH2,RHP2 (1 of 2) K06580 - ammonium transporter Rh (SLC42A, RHAG, RHBG, RHCG); Rhesus protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTTGGCAATCTGCGCGAGGCATACGCCGGGGCACATTCATACGTTCT
Internal bar code:CCGTGGGGGGTGTGTCGCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1854
LEAP-Seq percent confirming:31.25
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGAAGTTCCACAGCGGTG
Suggested primer 2:CTTCTTGCTTGTTTGCGGCT