Insertion junction: CLIP2.004680_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.004680
Chromosome:chromosome 7
Location:31769
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g312050 (1 of 1) 1.5.3.1//1.5.3.7 - Sarcosine oxidase // L-pipecolate oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCACAGTGGAGAACCGTGCTGCCGACGTGGCCGTGCTGGGCTCCAGCA
Internal bar code:TACCCTGATGCGGATTTGGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:5991
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:113
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:113

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAACATCCATCGGGTGTT
Suggested primer 2:AAGCCAACCAATTGCGGAAC