| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004680 |
| Chromosome: | chromosome 7 |
| Location: | 31769 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312050 | (1 of 1) 1.5.3.1//1.5.3.7 - Sarcosine oxidase // L-pipecolate oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTGCTACTGCCAGGGTTGGTGATCAGCAGGGAGGGACAAGCTACGTC |
| Internal bar code: | GGGTTCCAGAGTATCTTCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4845 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCAACCAATTGCGGAAC |
| Suggested primer 2: | GGGAACATCCATCGGGTGTT |