Insertion junction: CLIP2.004681_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004681
Chromosome:chromosome 6
Location:2902269
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272850 uL10m,MRPL10,PRPL10 (1 of 1) K02864 - large subunit ribosomal protein L10 (RP-L10, MRPL10, rplJ); Mitochondrial/chloroplast ribosomal protein L10 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCCAGCCCTTATCCTACCAACAAACCTATCTCCTGTCAAACGAGCTC
Internal bar code:AATTTGTAGGTGGTCGTCCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2158
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACGACGACAAGACCAAG
Suggested primer 2:GGCTGGTGCTGGAGTGTTAT