| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004694 |
| Chromosome: | chromosome 4 |
| Location: | 385422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217911 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGGAAAGGAGACGAACCTGGATGGTGGCCCCGCCTCCCACTGAATT |
| Internal bar code: | ATGCACCCAGTCTCTTGCTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2042 |
| LEAP-Seq percent confirming: | 94.5455 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTTATGGTCGTATCCCA |
| Suggested primer 2: | GGTACTCTTGTGACACGCCA |