| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004695 |
| Chromosome: | chromosome 12 |
| Location: | 2354111 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507950 | CGL98 | possible permease; (1 of 1) PTHR22911//PTHR22911:SF60 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGAGTTCTTACCAGAGGCCACATGGCTGTCGTTCTGCAAGCCCGGC |
| Internal bar code: | TAAGCCATATCCTCAGACCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1534 |
| LEAP-Seq percent confirming: | 81.3953 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTGGCTGGGTTTTGCAG |
| Suggested primer 2: | AAGGCTTTAGGTCGGCGTAC |