Insertion junction: CLIP2.004697_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.004697
Chromosome:chromosome 13
Location:4824789
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGCGCGTGTGTGGAATGAAGGTGTGCGCGTGTGTGCGTGCTAGCTAA
Internal bar code:GCTCGTCTTGGAACTGTGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1173
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTTCCTCGTGATCGTCCT
Suggested primer 2:GCACTGTGGCTACTACTGCA