Insertion junction: CLIP2.004706_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004706
Chromosome:chromosome 11
Location:1580982
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g468377 (1 of 1) K12602 - WD repeat-containing protein 61 (WDR61, REC14, SKI8) 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCGATTGCTTGGTTTAAGGCCCCCCGGCTTGCGGCACTTATCCTCTG
Internal bar code:TAACCGATCACCCGTTTCATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4208
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TACATGTTGAAGCGCCCACT
Suggested primer 2:AACATCGCACCTTCGGAAGT