Insertion junction: CLIP2.004718_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004718
Chromosome:chromosome 6
Location:1637865
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261900 FAP71 (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt); Flagellar Associated Protein 71 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTCGGCCATCTGCCCCATACTTCGGTTTGCGCGCTCGGCACCCACCTT
Internal bar code:CTTTCCTTATATTGCGACGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCCAAACACAACACCGC
Suggested primer 2:CACACACGCGCATCATACTG