| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004724 |
| Chromosome: | chromosome 9 |
| Location: | 6086696 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412803 | CGL88 | predicted protein; (1 of 1) PTHR10383//PTHR10383:SF24 - SERINE INCORPORATOR // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACACGAGCCAGTCCTGCAGAGAGGGACACGCCCGATGCGAAACGCA |
| Internal bar code: | CTTACTGGTAAATTTCGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1252 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACGCAATCAGATGTGAG |
| Suggested primer 2: | CCATATCGACCTGCGTCACA |