Insertion junction: CLIP2.004750_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004750
Chromosome:chromosome 1
Location:5622046
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040150 (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGCGCGCAGTGTCGCCACCCATCGAACCCAACAGCAGCAGCTGCTCA
Internal bar code:TTAATGCATGTGCTCGCTACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3969
LEAP-Seq percent confirming:95.5556
LEAP-Seq n confirming:86
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:CATCATCCATCGCGACCTCA
Suggested primer 2:AGTGTTATTGCCGCTCACGA