| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004764 |
| Chromosome: | chromosome 12 |
| Location: | 4331527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518950 | PGM13 | (1 of 2) PTHR23029//PTHR23029:SF12 - PHOSPHOGLYCERATE MUTASE // PHOSPHOMUTASE PMU1-RELATED; Phosphoglycerate mutase | 3'UTR |
| Cre12.g519000 | ASB2,PAI1 | Phosphoribosylanthranilate isomerase 1; (1 of 1) 5.3.1.24 - Phosphoribosylanthranilate isomerase / PRAI | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTAGAAATCTTCTTGACCGGTACAGTGAGCTCAGTCTACTACATTG |
| Internal bar code: | CTTGGTGAGGTGCATCGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1345 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACCGCGCCTTACTATA |
| Suggested primer 2: | GCACAAGTGGTACGAGGTGA |