| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004772 |
| Chromosome: | chromosome 9 |
| Location: | 3197963 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394584 | GID2 | Methyltransferase possibly involved in ribosome biogenesis; (1 of 1) 2.1.1.170 - 16S rRNA (guanine(527)-N(7))-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAAACCGAGACTTTGCTTCCGCTTCTGACTCCAGCAAACCCTTACAC |
| Internal bar code: | TTAGGTGTGTCGACAAATGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2739 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGAGGGATTGCTGAATTG |
| Suggested primer 2: | CAGAGCTGGATGCATCGCTA |