| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004774 |
| Chromosome: | chromosome 3 |
| Location: | 2473845 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159150 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 5'UTR | |
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAAAGAATTCCTGACAGGGATTGACGACGTAACTGGAGGATGGGTGCC |
| Internal bar code: | GGTCTTTTGTAGATTCGAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4212 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGGTCGTAAGGAGGTC |
| Suggested primer 2: | CGAACAAGGAGTCACGACCA |