Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.004800 |
Chromosome: | chromosome 6 |
Location: | 2648738 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g270350 | AMYB2,AMB2 | Beta-amylase 2; (1 of 3) 3.2.1.2 - Beta-amylase / Saccharogen amylase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCGCATGTAGACTGCAGCTGAGCCCCATCGCCACCAATCCGAATTAG |
Internal bar code: | AAACTATAATGGTGTGTTTGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4669 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 52 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGCTCGTAGTTATGTCCGG |
Suggested primer 2: | GGCTGATGCAGTGTGTGTTG |