Insertion junction: CLIP2.004807_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004807
Chromosome:chromosome 2
Location:2978875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095350 DNK1,CGL116 Conserved in the Green Lineage; (1 of 1) PTHR10513 - DEOXYNUCLEOSIDE KINASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTCCAGGCGCTGCTTGAGTGTGACGGCCAGGGTGCAGGCGTCGCGCA
Internal bar code:TTGCGCGCTGACAGCGTCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:341
LEAP-Seq percent confirming:15.3846
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCATGTGGATACGGGGG
Suggested primer 2:CCAACATTACGACGTCCCCA