| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004825 |
| Chromosome: | chromosome 12 |
| Location: | 95171 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483950 | MDN4,MDH4 | (1 of 3) K00026 - malate dehydrogenase (MDH2); Malate dehydrogenase 4 | 3'UTR |
| Cre12.g484000 | BCX1,ACX1 | Beta-carboxyltransferase (ACCase complex); (1 of 1) K01963 - acetyl-CoA carboxylase carboxyl transferase subunit beta (accD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGACGTCGCGAGCCCGTGCACGGCCGCTGAGGCTAGCAGCACAGCTCG |
| Internal bar code: | ATTACTATAAAAATTTTCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3033 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGAAGGGTGTGCAGTTC |
| Suggested primer 2: | GTATACAGGGCGTGGAGTCG |