| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004829 |
| Chromosome: | chromosome 10 |
| Location: | 1663840 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429650 | (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAGGCAGCTTATGCACGCATGCATCTTGCTGTGGGCTACGCGCTTGC |
| Internal bar code: | TGCTATGTGATGTGGGAGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 13.6364 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATCCCAGACAGTGTGCA |
| Suggested primer 2: | CGTCACCTGATCGAGATGGG |