Insertion junction: CLIP2.004833_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.004833
Chromosome:chromosome 3
Location:2595667
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g160250 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTAGTAGGTCCACCTCAGGGTCAGACATGGCGAGGCTGTTCACTCGG
Internal bar code:ACTCAGTATACAATGTATCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1640
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGCCAAATTCGCCATCG
Suggested primer 2:CTCCTCCACAATGGCTACGG