Insertion junction: CLIP2.004834_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.004834
Chromosome:chromosome 10
Location:2793823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438650 ADK2 Adenylate kinase; (1 of 1) 2.7.4.14 - UMP/CMP kinase / Uridine monophosphate-cytidine monophosphate phosphotransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGCTCATTGACGGGTTTCCTCGCAACCACGAGAACCGGACTGTGTGG
Internal bar code:CTCTGTAGATTGCTGATCATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:866
LEAP-Seq percent confirming:36.8421
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACACACGAACTAGCCAC
Suggested primer 2:TACCTTTACTTGCACGCGGT