Insertion junction: CLIP2.004850_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004850
Chromosome:chromosome 3
Location:4146235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g172850 OASTL3,ASL3 (1 of 3) 2.5.1.47 - Cysteine synthase / OAS sulfhydrylase; O-acetylserine (Thiol)-lyase/cysteine synthase 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGATGGTGGTGGTGGTGGTGGTGGGATGCGGCGTAGGGGTTGCAGTCG
Internal bar code:AAGCACGTATTTGTTCCCGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:61.1111
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGACGGGATGTGTCAAC
Suggested primer 2:ACCCCATACACACACACGTC