Insertion junction: CLIP2.004869_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004869
Chromosome:chromosome 16
Location:5532508
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g682100 TDR1 (1 of 1) 1.1.1.206//1.1.1.236 - Tropinone reductase I / Tropinone reductase // Tropinone reductase II / Tropinone reductase; Tropine dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTATGTTCAGCACTGCGCGCGCTGTCCAACATAGGGAGGGCCAGGGGCC
Internal bar code:TGCCGGGCTTCTGCTTATGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:5570
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCGTTGTGAGGGTTTGTG
Suggested primer 2:CAGTAGGCACAGCAGGAACA