| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004876 |
| Chromosome: | chromosome 12 |
| Location: | 2206894 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509001 | RPK2 | RNA-polymerase-subunit kinase; (1 of 2) PTHR24055//PTHR24055:SF10 - MITOGEN-ACTIVATED PROTEIN KINASE // MITOGEN-ACTIVATED PROTEIN KINASE 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGGCGGATGCATCGTAGGCACTGCATCGCAGGTCCGCTCAGGGGCTC |
| Internal bar code: | AGTATGAAGGTTTGGAGTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1805 |
| LEAP-Seq percent confirming: | 95.7143 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCGCAGGAAAAATGCAA |
| Suggested primer 2: | GCGTGTAGGTCCCGTCATAG |