| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004883 |
| Chromosome: | chromosome 6 |
| Location: | 4226875 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278246 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATATGGAATGCATTACGGCATATGACTGAACAGCCCCTGTGATGAGT |
| Internal bar code: | GATCGCGCAATTAAGGTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1073 |
| LEAP-Seq percent confirming: | 82.6923 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTCAGTGGACATCTGGC |
| Suggested primer 2: | GGTGAAGGTGCCGTTAGTGA |