| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004893 |
| Chromosome: | chromosome 3 |
| Location: | 4041581 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171900 | FAP56 | Flagellar Associated Protein 56 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGCTCATCCCCACCTCGCCACGGTCCGTGGAGTCGTGCTTCCGGTT |
| Internal bar code: | CTTGGATCCACAGATATGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTCTGCTGCCTGTCCAG |
| Suggested primer 2: | TCCTCACTTCACTGCGGTTC |