| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004894 |
| Chromosome: | chromosome 3 |
| Location: | 622237 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145507 | (1 of 1) IPR004720 - Phosphotransferase system, sorbose subfamily IIB component | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCATGTAGGAGGCTGAGTGGTGTGTGCGTGTGTGAATGAGAGAGAAA |
| Internal bar code: | CTTGTATGTGCCCTTGGCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2133 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGCCGCTTATGCAATGG |
| Suggested primer 2: | TCGCAGTTCAACCTACACCC |